Characterization of magnesium doped sol-gel biomaterial for bone tissue regeneration: The effect of Mg ion in protein adsorption

Magnesium is the fourth most abundant element in human body with a wide battery of functions maintenance normal cell homeostasis. In bone, this incorporates hydroxyapatite structure and it takes part mineral metabolism regulates osteoclast functions. study, sol-gel materials increasing concentrations MgCl 2 (0.5, 1, 1.5%) were synthesized applied onto Ti surfaces as coatings. The first physicochemically characterized. vitro responses examined using MC3T3-E1 osteoblastic cells RAW264.7 macrophages. Human serum protein adsorption was evaluated employing nLC-MS/MS. incorporation Mg did not affect crosslinking network, controlled release observed; cytotoxic at any tested concentrations. cytoskeleton arrangement cultured on Mg-doped changed comparison controls; became more elongated, protruded lamellipodia increased surface. expression integrins (ITGA5 ITGB1) boosted by Mg-coatings. ALP activity TGF-?, OSX RUNX2 genes also increased. cells, TNF-? secretion reduced, while TGF-? IL-4 rose. These changes correlated altered patterns. coatings showed anti-inflammatory (CLUS, IC1, CFAH, VTNC), adhesion (DSG1, FILA2, DESP) tissue regeneration (VTNC CYTA) proteins. This integrated approach to biomaterial characterization revealed potential bone regeneration. • New Mg-enriched osteogenic proprieties. Osteoblast increases incorporate into proteomic report effect biomaterials. Protein patterns Mg-coatings correlates their results.